Knock-In:Article Title: Functional rescue of a disease-linked ERAD pathway mutation via alternative splicing.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models C57BL/6J (M. musculus) (Knockin) The University of Michigan Molecular Genetics Core HEK293T ATCC CRL-3216 Human Fibroblast Weis Lab (Weis et al, 2024) 8 The EMBO Journal © The Author(s) Reagent/resource Reference or source Identifier or catalog number Recombinant DNA pcDNA5-GFP-IL7R-XbaI mutated minigene construct Kuyumcu-Martinez Lab (Belanger et al, 2018) pcDNA5-GFP-SEL1Lexon4 GenScript Biotech Fig. 3C Antibodies Rabbit anti-CD19 Cell Signaling Technology 90176S Mouse anti-SATB2 Abcam ab51502 Rat anti-CTIP2 Abcam ab18465 Alexa Fluor 555-conjugated donkey anti-rabbit IgG Jackson ImmunoResearch 711-565-152 Alexa Fluor 488-conjugated goat anti-rat IgG Invitrogen A-11006 Alexa Fluor 555-conjugated donkey anti-mouse IgG Invitrogen A32773 Mouse anti-HSP90 Santa Cruz sc-13119 Rabbit anti-SEL1L home-made (Zhou et al, 2020) Rabbit anti-SEL1L Abcam ab78298 Rabbit anti-HRD1 Proteintech 13473-1 Rabbit anti-CD147 Proteintech 11989-1 Rabbit anti-IRE1α Cell Signaling Technology 3294 Rabbit anti-ERLEC1 Abcam ab181166 Rabbit anti-BiP/GRP94 Abcam ab21685 Rabbit anti-PDI Enzo ADI-SPA-890 Rabbit anti-PERK Cell Signaling Technology 3192 Anti-Rabbit IgG TrueBlot HRP Rockland 18-8816-33 Anti-Mouse IgG TrueBlot-HRP Rockland 18-8817-31 IgG Cell Signaling Technology 2729S ZombieNIR Fixable Viability dye BioLegend 423106 Rat anti-mouse CD16/CD32 eBioscience Ebio 14-0161- 85 Rat anti-CD45 (30-F11), eBioscience Ebio48-403182 Rat anti-CD45R/B220 (RA36B2) BioLegend 103208 Rat anti-CD19 (6D5) BioLegend 115512 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S1 Sequencing primers This study Appendix Table S1 Antisense oligonucleotides This study Appendix Table S1 Chemicals, enzymes and other reagents Tunicamycin Tocris 3516 PBS Corning 21-040-CV 10% Neutral Buffered Formalin VWR® Histology Reagents 89370-094 Sucrose Sigma-Aldrich SO389 Reagent/resource Reference or source Identifier or catalog number Tissue-Tek® O.C.T. .. Compound Sakura Finetek USA Inc 4583 Normal Donkey Serum Jackson ImmunoResearch 017-000-121 Tween-20 Sigma-Aldrich P1379-6X1L ProLongTM Diamond Antifade Mountant with DAPI Fisher Scientific P36971 TRI Reagent and BCP phase separation reagent Molecular Research Center TR 118 Protease inhibitor Sigma-Aldrich P8340 DTT Fisher Scientific BP172-5 Phosphatase inhibitor cocktail Bio Basic PL017 Bio-Rad Protein Assay Dye Bio-Rad 5000006 Phos-tagTM Acrylamide Wako Chemicals 304-93521 Thapsigargin Tocris 1138 EndoH ACROBiosystems ENH-S5116 PNGase F New England BioLabs P0704L Endo-Porter Morpholino Gene Tool, LLC OT-EP-PEG-1 16% Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences 15710 EDTA, 0.5M, pH 8.0, Molecular Biology Grade, DEPC-Treated Sigma-Aldrich 324506 Protein A agarose beads Invitrogen 15918-014 30% Acrylamide/Bis Solution, 29:1 Bio-Rad 1610156 40% Acrylamide/Bis Solution, 19:1 Bio-Rad 1610144 HotStart Taq 2X PCR Master Mix with Dye ABclonal RK20605 Agarose (Low-EEO/MultiPurpose/Molecular Biology Grade) Fisher BioReagents BP160-500 Rabbit TrueBlot®: Anti-Rabbit IgG HRP Rockland 18-8816-33 SuperScriptTM III Reverse Transcriptase Invitrogen 18080093 RNaseOUTTM Recombinant Ribonuclease Inhibitor Invitrogen 10777019 Methanol (Certified ACS), Fisher Chemical Fisher Scientific A412-4 GibcoTM DMEM, high glucose, pyruvate Gibco 11-995-081 Trypsin-EDTA (0.25%), phenol red Gibco 25200114 MycoStripTM - Mycoplasma Detection Kit Invivogen rep-mys-20 Software ImageJ FIJI software FCS Express 7 Research De Novo Software Image Lab Bio-Rad SnapGene SnapGene © The Author(s) The EMBO Journal 9 Reagent/resource Reference or source Identifier or catalog number Other Leica DMi8 THUNDER Imager Leica CBS Scientific Adjustable Height Vertical Gel System C.B.S.
Recombinant:Article Title: Functional rescue of a disease-linked ERAD pathway mutation via alternative splicing.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models C57BL/6J (M. musculus) (Knockin) The University of Michigan Molecular Genetics Core HEK293T ATCC CRL-3216 Human Fibroblast Weis Lab (Weis et al, 2024) 8 The EMBO Journal © The Author(s) Reagent/resource Reference or source Identifier or catalog number Recombinant DNA pcDNA5-GFP-IL7R-XbaI mutated minigene construct Kuyumcu-Martinez Lab (Belanger et al, 2018) pcDNA5-GFP-SEL1Lexon4 GenScript Biotech Fig. 3C Antibodies Rabbit anti-CD19 Cell Signaling Technology 90176S Mouse anti-SATB2 Abcam ab51502 Rat anti-CTIP2 Abcam ab18465 Alexa Fluor 555-conjugated donkey anti-rabbit IgG Jackson ImmunoResearch 711-565-152 Alexa Fluor 488-conjugated goat anti-rat IgG Invitrogen A-11006 Alexa Fluor 555-conjugated donkey anti-mouse IgG Invitrogen A32773 Mouse anti-HSP90 Santa Cruz sc-13119 Rabbit anti-SEL1L home-made (Zhou et al, 2020) Rabbit anti-SEL1L Abcam ab78298 Rabbit anti-HRD1 Proteintech 13473-1 Rabbit anti-CD147 Proteintech 11989-1 Rabbit anti-IRE1α Cell Signaling Technology 3294 Rabbit anti-ERLEC1 Abcam ab181166 Rabbit anti-BiP/GRP94 Abcam ab21685 Rabbit anti-PDI Enzo ADI-SPA-890 Rabbit anti-PERK Cell Signaling Technology 3192 Anti-Rabbit IgG TrueBlot HRP Rockland 18-8816-33 Anti-Mouse IgG TrueBlot-HRP Rockland 18-8817-31 IgG Cell Signaling Technology 2729S ZombieNIR Fixable Viability dye BioLegend 423106 Rat anti-mouse CD16/CD32 eBioscience Ebio 14-0161- 85 Rat anti-CD45 (30-F11), eBioscience Ebio48-403182 Rat anti-CD45R/B220 (RA36B2) BioLegend 103208 Rat anti-CD19 (6D5) BioLegend 115512 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S1 Sequencing primers This study Appendix Table S1 Antisense oligonucleotides This study Appendix Table S1 Chemicals, enzymes and other reagents Tunicamycin Tocris 3516 PBS Corning 21-040-CV 10% Neutral Buffered Formalin VWR® Histology Reagents 89370-094 Sucrose Sigma-Aldrich SO389 Reagent/resource Reference or source Identifier or catalog number Tissue-Tek® O.C.T. .. Compound Sakura Finetek USA Inc 4583 Normal Donkey Serum Jackson ImmunoResearch 017-000-121 Tween-20 Sigma-Aldrich P1379-6X1L ProLongTM Diamond Antifade Mountant with DAPI Fisher Scientific P36971 TRI Reagent and BCP phase separation reagent Molecular Research Center TR 118 Protease inhibitor Sigma-Aldrich P8340 DTT Fisher Scientific BP172-5 Phosphatase inhibitor cocktail Bio Basic PL017 Bio-Rad Protein Assay Dye Bio-Rad 5000006 Phos-tagTM Acrylamide Wako Chemicals 304-93521 Thapsigargin Tocris 1138 EndoH ACROBiosystems ENH-S5116 PNGase F New England BioLabs P0704L Endo-Porter Morpholino Gene Tool, LLC OT-EP-PEG-1 16% Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences 15710 EDTA, 0.5M, pH 8.0, Molecular Biology Grade, DEPC-Treated Sigma-Aldrich 324506 Protein A agarose beads Invitrogen 15918-014 30% Acrylamide/Bis Solution, 29:1 Bio-Rad 1610156 40% Acrylamide/Bis Solution, 19:1 Bio-Rad 1610144 HotStart Taq 2X PCR Master Mix with Dye ABclonal RK20605 Agarose (Low-EEO/MultiPurpose/Molecular Biology Grade) Fisher BioReagents BP160-500 Rabbit TrueBlot®: Anti-Rabbit IgG HRP Rockland 18-8816-33 SuperScriptTM III Reverse Transcriptase Invitrogen 18080093 RNaseOUTTM Recombinant Ribonuclease Inhibitor Invitrogen 10777019 Methanol (Certified ACS), Fisher Chemical Fisher Scientific A412-4 GibcoTM DMEM, high glucose, pyruvate Gibco 11-995-081 Trypsin-EDTA (0.25%), phenol red Gibco 25200114 MycoStripTM - Mycoplasma Detection Kit Invivogen rep-mys-20 Software ImageJ FIJI software FCS Express 7 Research De Novo Software Image Lab Bio-Rad SnapGene SnapGene © The Author(s) The EMBO Journal 9 Reagent/resource Reference or source Identifier or catalog number Other Leica DMi8 THUNDER Imager Leica CBS Scientific Adjustable Height Vertical Gel System C.B.S.
Construct:Article Title: Functional rescue of a disease-linked ERAD pathway mutation via alternative splicing.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models C57BL/6J (M. musculus) (Knockin) The University of Michigan Molecular Genetics Core HEK293T ATCC CRL-3216 Human Fibroblast Weis Lab (Weis et al, 2024) 8 The EMBO Journal © The Author(s) Reagent/resource Reference or source Identifier or catalog number Recombinant DNA pcDNA5-GFP-IL7R-XbaI mutated minigene construct Kuyumcu-Martinez Lab (Belanger et al, 2018) pcDNA5-GFP-SEL1Lexon4 GenScript Biotech Fig. 3C Antibodies Rabbit anti-CD19 Cell Signaling Technology 90176S Mouse anti-SATB2 Abcam ab51502 Rat anti-CTIP2 Abcam ab18465 Alexa Fluor 555-conjugated donkey anti-rabbit IgG Jackson ImmunoResearch 711-565-152 Alexa Fluor 488-conjugated goat anti-rat IgG Invitrogen A-11006 Alexa Fluor 555-conjugated donkey anti-mouse IgG Invitrogen A32773 Mouse anti-HSP90 Santa Cruz sc-13119 Rabbit anti-SEL1L home-made (Zhou et al, 2020) Rabbit anti-SEL1L Abcam ab78298 Rabbit anti-HRD1 Proteintech 13473-1 Rabbit anti-CD147 Proteintech 11989-1 Rabbit anti-IRE1α Cell Signaling Technology 3294 Rabbit anti-ERLEC1 Abcam ab181166 Rabbit anti-BiP/GRP94 Abcam ab21685 Rabbit anti-PDI Enzo ADI-SPA-890 Rabbit anti-PERK Cell Signaling Technology 3192 Anti-Rabbit IgG TrueBlot HRP Rockland 18-8816-33 Anti-Mouse IgG TrueBlot-HRP Rockland 18-8817-31 IgG Cell Signaling Technology 2729S ZombieNIR Fixable Viability dye BioLegend 423106 Rat anti-mouse CD16/CD32 eBioscience Ebio 14-0161- 85 Rat anti-CD45 (30-F11), eBioscience Ebio48-403182 Rat anti-CD45R/B220 (RA36B2) BioLegend 103208 Rat anti-CD19 (6D5) BioLegend 115512 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S1 Sequencing primers This study Appendix Table S1 Antisense oligonucleotides This study Appendix Table S1 Chemicals, enzymes and other reagents Tunicamycin Tocris 3516 PBS Corning 21-040-CV 10% Neutral Buffered Formalin VWR® Histology Reagents 89370-094 Sucrose Sigma-Aldrich SO389 Reagent/resource Reference or source Identifier or catalog number Tissue-Tek® O.C.T. .. Compound Sakura Finetek USA Inc 4583 Normal Donkey Serum Jackson ImmunoResearch 017-000-121 Tween-20 Sigma-Aldrich P1379-6X1L ProLongTM Diamond Antifade Mountant with DAPI Fisher Scientific P36971 TRI Reagent and BCP phase separation reagent Molecular Research Center TR 118 Protease inhibitor Sigma-Aldrich P8340 DTT Fisher Scientific BP172-5 Phosphatase inhibitor cocktail Bio Basic PL017 Bio-Rad Protein Assay Dye Bio-Rad 5000006 Phos-tagTM Acrylamide Wako Chemicals 304-93521 Thapsigargin Tocris 1138 EndoH ACROBiosystems ENH-S5116 PNGase F New England BioLabs P0704L Endo-Porter Morpholino Gene Tool, LLC OT-EP-PEG-1 16% Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences 15710 EDTA, 0.5M, pH 8.0, Molecular Biology Grade, DEPC-Treated Sigma-Aldrich 324506 Protein A agarose beads Invitrogen 15918-014 30% Acrylamide/Bis Solution, 29:1 Bio-Rad 1610156 40% Acrylamide/Bis Solution, 19:1 Bio-Rad 1610144 HotStart Taq 2X PCR Master Mix with Dye ABclonal RK20605 Agarose (Low-EEO/MultiPurpose/Molecular Biology Grade) Fisher BioReagents BP160-500 Rabbit TrueBlot®: Anti-Rabbit IgG HRP Rockland 18-8816-33 SuperScriptTM III Reverse Transcriptase Invitrogen 18080093 RNaseOUTTM Recombinant Ribonuclease Inhibitor Invitrogen 10777019 Methanol (Certified ACS), Fisher Chemical Fisher Scientific A412-4 GibcoTM DMEM, high glucose, pyruvate Gibco 11-995-081 Trypsin-EDTA (0.25%), phenol red Gibco 25200114 MycoStripTM - Mycoplasma Detection Kit Invivogen rep-mys-20 Software ImageJ FIJI software FCS Express 7 Research De Novo Software Image Lab Bio-Rad SnapGene SnapGene © The Author(s) The EMBO Journal 9 Reagent/resource Reference or source Identifier or catalog number Other Leica DMi8 THUNDER Imager Leica CBS Scientific Adjustable Height Vertical Gel System C.B.S.
Sequencing:Article Title: Functional rescue of a disease-linked ERAD pathway mutation via alternative splicing.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models C57BL/6J (M. musculus) (Knockin) The University of Michigan Molecular Genetics Core HEK293T ATCC CRL-3216 Human Fibroblast Weis Lab (Weis et al, 2024) 8 The EMBO Journal © The Author(s) Reagent/resource Reference or source Identifier or catalog number Recombinant DNA pcDNA5-GFP-IL7R-XbaI mutated minigene construct Kuyumcu-Martinez Lab (Belanger et al, 2018) pcDNA5-GFP-SEL1Lexon4 GenScript Biotech Fig. 3C Antibodies Rabbit anti-CD19 Cell Signaling Technology 90176S Mouse anti-SATB2 Abcam ab51502 Rat anti-CTIP2 Abcam ab18465 Alexa Fluor 555-conjugated donkey anti-rabbit IgG Jackson ImmunoResearch 711-565-152 Alexa Fluor 488-conjugated goat anti-rat IgG Invitrogen A-11006 Alexa Fluor 555-conjugated donkey anti-mouse IgG Invitrogen A32773 Mouse anti-HSP90 Santa Cruz sc-13119 Rabbit anti-SEL1L home-made (Zhou et al, 2020) Rabbit anti-SEL1L Abcam ab78298 Rabbit anti-HRD1 Proteintech 13473-1 Rabbit anti-CD147 Proteintech 11989-1 Rabbit anti-IRE1α Cell Signaling Technology 3294 Rabbit anti-ERLEC1 Abcam ab181166 Rabbit anti-BiP/GRP94 Abcam ab21685 Rabbit anti-PDI Enzo ADI-SPA-890 Rabbit anti-PERK Cell Signaling Technology 3192 Anti-Rabbit IgG TrueBlot HRP Rockland 18-8816-33 Anti-Mouse IgG TrueBlot-HRP Rockland 18-8817-31 IgG Cell Signaling Technology 2729S ZombieNIR Fixable Viability dye BioLegend 423106 Rat anti-mouse CD16/CD32 eBioscience Ebio 14-0161- 85 Rat anti-CD45 (30-F11), eBioscience Ebio48-403182 Rat anti-CD45R/B220 (RA36B2) BioLegend 103208 Rat anti-CD19 (6D5) BioLegend 115512 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S1 Sequencing primers This study Appendix Table S1 Antisense oligonucleotides This study Appendix Table S1 Chemicals, enzymes and other reagents Tunicamycin Tocris 3516 PBS Corning 21-040-CV 10% Neutral Buffered Formalin VWR® Histology Reagents 89370-094 Sucrose Sigma-Aldrich SO389 Reagent/resource Reference or source Identifier or catalog number Tissue-Tek® O.C.T. .. Compound Sakura Finetek USA Inc 4583 Normal Donkey Serum Jackson ImmunoResearch 017-000-121 Tween-20 Sigma-Aldrich P1379-6X1L ProLongTM Diamond Antifade Mountant with DAPI Fisher Scientific P36971 TRI Reagent and BCP phase separation reagent Molecular Research Center TR 118 Protease inhibitor Sigma-Aldrich P8340 DTT Fisher Scientific BP172-5 Phosphatase inhibitor cocktail Bio Basic PL017 Bio-Rad Protein Assay Dye Bio-Rad 5000006 Phos-tagTM Acrylamide Wako Chemicals 304-93521 Thapsigargin Tocris 1138 EndoH ACROBiosystems ENH-S5116 PNGase F New England BioLabs P0704L Endo-Porter Morpholino Gene Tool, LLC OT-EP-PEG-1 16% Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences 15710 EDTA, 0.5M, pH 8.0, Molecular Biology Grade, DEPC-Treated Sigma-Aldrich 324506 Protein A agarose beads Invitrogen 15918-014 30% Acrylamide/Bis Solution, 29:1 Bio-Rad 1610156 40% Acrylamide/Bis Solution, 19:1 Bio-Rad 1610144 HotStart Taq 2X PCR Master Mix with Dye ABclonal RK20605 Agarose (Low-EEO/MultiPurpose/Molecular Biology Grade) Fisher BioReagents BP160-500 Rabbit TrueBlot®: Anti-Rabbit IgG HRP Rockland 18-8816-33 SuperScriptTM III Reverse Transcriptase Invitrogen 18080093 RNaseOUTTM Recombinant Ribonuclease Inhibitor Invitrogen 10777019 Methanol (Certified ACS), Fisher Chemical Fisher Scientific A412-4 GibcoTM DMEM, high glucose, pyruvate Gibco 11-995-081 Trypsin-EDTA (0.25%), phenol red Gibco 25200114 MycoStripTM - Mycoplasma Detection Kit Invivogen rep-mys-20 Software ImageJ FIJI software FCS Express 7 Research De Novo Software Image Lab Bio-Rad SnapGene SnapGene © The Author(s) The EMBO Journal 9 Reagent/resource Reference or source Identifier or catalog number Other Leica DMi8 THUNDER Imager Leica CBS Scientific Adjustable Height Vertical Gel System C.B.S.
Polymerase Chain Reaction:Article Title: Functional rescue of a disease-linked ERAD pathway mutation via alternative splicing.
Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalog number Experimental models C57BL/6J (M. musculus) (Knockin) The University of Michigan Molecular Genetics Core HEK293T ATCC CRL-3216 Human Fibroblast Weis Lab (Weis et al, 2024) 8 The EMBO Journal © The Author(s) Reagent/resource Reference or source Identifier or catalog number Recombinant DNA pcDNA5-GFP-IL7R-XbaI mutated minigene construct Kuyumcu-Martinez Lab (Belanger et al, 2018) pcDNA5-GFP-SEL1Lexon4 GenScript Biotech Fig. 3C Antibodies Rabbit anti-CD19 Cell Signaling Technology 90176S Mouse anti-SATB2 Abcam ab51502 Rat anti-CTIP2 Abcam ab18465 Alexa Fluor 555-conjugated donkey anti-rabbit IgG Jackson ImmunoResearch 711-565-152 Alexa Fluor 488-conjugated goat anti-rat IgG Invitrogen A-11006 Alexa Fluor 555-conjugated donkey anti-mouse IgG Invitrogen A32773 Mouse anti-HSP90 Santa Cruz sc-13119 Rabbit anti-SEL1L home-made (Zhou et al, 2020) Rabbit anti-SEL1L Abcam ab78298 Rabbit anti-HRD1 Proteintech 13473-1 Rabbit anti-CD147 Proteintech 11989-1 Rabbit anti-IRE1α Cell Signaling Technology 3294 Rabbit anti-ERLEC1 Abcam ab181166 Rabbit anti-BiP/GRP94 Abcam ab21685 Rabbit anti-PDI Enzo ADI-SPA-890 Rabbit anti-PERK Cell Signaling Technology 3192 Anti-Rabbit IgG TrueBlot HRP Rockland 18-8816-33 Anti-Mouse IgG TrueBlot-HRP Rockland 18-8817-31 IgG Cell Signaling Technology 2729S ZombieNIR Fixable Viability dye BioLegend 423106 Rat anti-mouse CD16/CD32 eBioscience Ebio 14-0161- 85 Rat anti-CD45 (30-F11), eBioscience Ebio48-403182 Rat anti-CD45R/B220 (RA36B2) BioLegend 103208 Rat anti-CD19 (6D5) BioLegend 115512 Oligonucleotides and other sequence-based reagents PCR primers This study Appendix Table S1 Sequencing primers This study Appendix Table S1 Antisense oligonucleotides This study Appendix Table S1 Chemicals, enzymes and other reagents Tunicamycin Tocris 3516 PBS Corning 21-040-CV 10% Neutral Buffered Formalin VWR® Histology Reagents 89370-094 Sucrose Sigma-Aldrich SO389 Reagent/resource Reference or source Identifier or catalog number Tissue-Tek® O.C.T. .. Compound Sakura Finetek USA Inc 4583 Normal Donkey Serum Jackson ImmunoResearch 017-000-121 Tween-20 Sigma-Aldrich P1379-6X1L ProLongTM Diamond Antifade Mountant with DAPI Fisher Scientific P36971 TRI Reagent and BCP phase separation reagent Molecular Research Center TR 118 Protease inhibitor Sigma-Aldrich P8340 DTT Fisher Scientific BP172-5 Phosphatase inhibitor cocktail Bio Basic PL017 Bio-Rad Protein Assay Dye Bio-Rad 5000006 Phos-tagTM Acrylamide Wako Chemicals 304-93521 Thapsigargin Tocris 1138 EndoH ACROBiosystems ENH-S5116 PNGase F New England BioLabs P0704L Endo-Porter Morpholino Gene Tool, LLC OT-EP-PEG-1 16% Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences 15710 EDTA, 0.5M, pH 8.0, Molecular Biology Grade, DEPC-Treated Sigma-Aldrich 324506 Protein A agarose beads Invitrogen 15918-014 30% Acrylamide/Bis Solution, 29:1 Bio-Rad 1610156 40% Acrylamide/Bis Solution, 19:1 Bio-Rad 1610144 HotStart Taq 2X PCR Master Mix with Dye ABclonal RK20605 Agarose (Low-EEO/MultiPurpose/Molecular Biology Grade) Fisher BioReagents BP160-500 Rabbit TrueBlot®: Anti-Rabbit IgG HRP Rockland 18-8816-33 SuperScriptTM III Reverse Transcriptase Invitrogen 18080093 RNaseOUTTM Recombinant Ribonuclease Inhibitor Invitrogen 10777019 Methanol (Certified ACS), Fisher Chemical Fisher Scientific A412-4 GibcoTM DMEM, high glucose, pyruvate Gibco 11-995-081 Trypsin-EDTA (0.25%), phenol red Gibco 25200114 MycoStripTM - Mycoplasma Detection Kit Invivogen rep-mys-20 Software ImageJ FIJI software FCS Express 7 Research De Novo Software Image Lab Bio-Rad SnapGene SnapGene © The Author(s) The EMBO Journal 9 Reagent/resource Reference or source Identifier or catalog number Other Leica DMi8 THUNDER Imager Leica CBS Scientific Adjustable Height Vertical Gel System C.B.S.
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